-by Kyoto Monotech-

Fast and Easy Antibody Purification with Monolith Technology

  1. By immobilizing protein A or protein G on the plate-shaped monolithic silica surface, antibody purification has become possible in a short period of time.
  2. Purification and concentration of the target IgG antibody and measurement of IgG antibody concentration in the culture medium can be easily performed.
  3. There are 4 types of shape.
  4. Each has two modified types, protein A and protein G.
Shape　(Monolith dimensions shown below)ligandspecificationpart numberprice
Ex-Pure Spin

ProtainA(ProA)
  • gel volume : φ4.8mm X 1.5mm = 27μL
  • binding capacity(human IgG )= 0.4mg/column
  • Maximum sample volume = 0.5mL
  • quantity=10pieces
  • Kit contents = column
    Various solutions (adsorption, washing, elution, regeneration, neutralization)
※Antibody purification is completed in about 2 minutes even with a desktop mini centrifuge. 		EP-A01JPY 18,000
ProtainG(ProG)EP-G01JPY 20,000
Ex-Pure 96well

ProtainＡ(ProA)
  • gel volume : φ4.8mm X 1.5mm = 27μL x 96個
  • >binding capacity(human IgG )= 0.4mg/column
  • Maximum sample volume = 0.5mL x 96well
  • quantity = １plate
  • Kit contents = column, top and bottom caps
    Various solutions (adsorption, washing, elution, regeneration, neutralization)
※A flow rate of about 1 mL/min is possible under the condition of 0.02 MPa, and a recovery rate equivalent to that of a spin column can be obtained. 		EW-A01JPY 78,000
ProtainG(ProG)EW-G01JPY 88,000
Ex-Pure Cyringe

ProtainA(ProA)
  • gel volume = φ15.6mm X 5.0mm(1,000μL)
  • binding capacity(human IgG) = 16mg/column(ProA)
  • Maximum sample volume = 8mL
  • quantity = 1piece
  • Kit Contents = Column, Centrifugal Adapter
    (Solvent kit sold separately)
※Either centrifuge or suction method can be used. 		EY-A02JPY 80,000
ProtainG(ProG)EY-G02JPY 90,000
Ex-Pure-Funnel

(Click the image to go to the specification drawing)

ProtainA(ProA) only
  • gel volume = φ40mm X 8.0mm(10mL)
  • binding capacity(human IgG) = 150mg/column
  • maximum sanple Volume = 250mL
  • quantity - 1piece
  • Kit contents = column, φ45mm bottle, connection adapter (solvent kit sold separately)
Compared to working with equipment, it simplifies the work after preparation and cleanup, so it is popular with customers because it saves time. 		EF-A01Contact us
solvent kit
  • Kit contents = each solution for adsorption, washing, elution, regeneration, and neutralization (250 mL each)
EY-S01JPY 48,000

※　Solvents are sold separately for Ex-Pure-Cyringe and Ex-Pure-Funnel.　(EY-S01)

Application data (column performance for commercial antibody drugs)


※Modified protein G and high-density chemical bonding technology to silica surfaces were invented and developed by the National Institute of Advanced Industrial Science and Technology.
Using this technology, Kyoto Monotech develops and produces the monolithic silica column "Ex-Pure" for antibody purification.

Please be sure to read the instruction manual before using or considering.


Ex-Pure-Spin operation example

  • Antibodies can be purified in about 2 minutes using a tabletop centrifuge (eg, rotation speed = 6,000 rpm), and operation can be performed in a short time compared to other companies' products.

  • Centrifugation (6,000 rpm) can be performed immediately after injecting the sample and solvent without pretreatment (Fig. 1).

  • Samples can be concentrated and purified by increasing the number of sample injections in step 2 or by reducing the volume of elution solvent to 20 μL in step 4.

Spin column performance

Ex-Pure ProA（Protain A）Ex-Pure ProG（Protain G）

Fig.２
Fig.３
　A spin column with Ex-Pure ProA as a ligand can purify up to 0.4 mg of human IgG with a recovery rate of over 90%. (Figure 2)
In addition, it is possible to quantitatively measure the amount of protein up to 0.3 mg in each process. 		　As with Ex-Pure ProA, the spin column with Ex-Pure ProG as a ligand can also purify and recover simple antibodies, and it is possible to quantitatively measure the amount of protein in each process.
In addition, IgG3 type antibodies that cannot bind to Ex-Pure ProA can also be purified and collected.

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