Ex-Pure　Extra data

Evaluation of antibody recovery accuracy (variation) of spin columns
  • Each spin column was randomly selected, 0.4 mL of 2.0 mg/mL human IgG was injected, and the washing → elution process was performed.
  • The table below shows the results of measuring the amount of antibody in the eluate using the UV method (A280) and the BCA method. (EX-Pure ProG only UV method)
Ex-Pure ProAEx-Pure ProG
UV法0.665±0.027mg/column0.504±0.056mg/column
BCA法0.641±0.079mg/column　　　　　NO DATA

Evaluation of antibody purification from culture medium by SDS-PAGE electrophoresis
This is the SDS-PAGE result when the antibody was actually purified from the culture medium with Ex-Pure.
Contaminants are removed in the flow-through solution in lane 3 and the washing solution in lane 4.