Technology

“Monolith Column” is another word for Kyoto Monotech . It is an evolutional column for liquid chromatography which breaks the common sense of column. It has been invented by Professor
(at the time) Naohiro Soga and Professor Kazuki Nakanishi (Kyoto University), and has been developed by Kyoto Monotech .

Monolith column structure

Unlike conventional columns packed with porous spherical particles, these monolith columns for liquid chromatography are available in rod-type (rod-shaped) or disc-type (disk-shaped; incorporated into spin columns, etc.) and use monolithic silica gel as a separation agent. These columns have two different pore sizes: macropores formed by a three-dimensional network structure, and mesopores, which result from the aggregate structure of the particles themselves.

　If you look at the cross section of monolithic silica gel with an SEM (electron microscope) image, you can see two types of pores: nanometer (nm) size (mesopores) and micrometer (μm) size (macropores).


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　It has received numerous awards both in Japan and overseas as an ultra-high-speed, high-resolution column for its high-speed, high-separation performance brought about by its thin framework and large flow path.
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Conventional particle packed columnHigh separation BUT･･･ High-back pressure
Monolith Column（Integrated type）　　　　　High separation　AND→　Low back pressure!!

　When monolithic silica gel is used for a column of liquid chromatography, analysis can be performed with
low-back pressure at high separation effeiciency. It also enables separation of various materials such as DNA
and proteins by using a tabletop type small centrifuge or pipette in several ten seconds.
In other words:

  1. You can control the mesopores and macropores and silica skeleton size independently.
  2. You can obtain high separation, low back pressure and easy operation at a same time.
  3. We will aim to simplify and cost reduction of the separation process.
  4. You can improve separation performance without changing the equipment you currently use.