MonoBis Capillary Column　－KYOTO MONOTECH －

MonoBis Capillary Column
Capillary MonoBis Capillary Column realizes ultra-high separation with its long column of column length 2,000mm and 4,000mm. 　Resolution at theoretical plates of 100,000 stage/m is guaranteed. 　Wherever you cut, you can obtain the same performance.
Cross-sectional view of MonoBis Capillary Column

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Guaranteed resolution of 10,000 theoretical plate Achieved 630,000 theoretical plates with a column length of 5,000 mm
  • Measurement condition　：　Load pressure is 30Mpa or less at length 5000mm
  • Mobile phase: 80% acetonitrile
  • Flow rate: 0.8 μL/min
  • Sample:
    1. Uracil
    2. Naphthylamine
    3. Naphthalene
    4. ethylbenzene
    5. Xylene
    6. propylbenzene
    7. butylbenzene
    8. o-Terphenyl
    9. Amylbenzene
    10. Triphenylene
Achieved 290,000 theoretical plates with a column length of 2,000 mm

　Analysis example of BA digest Gradient time：100 minutes Gradient time：300 minutes
Mesurement condition
  • column length　：　2,000mm
  • HPLC machine　　：　Prominence nano flow system
  • Flow rate 　：　500nL/min
  • Mobile phase：　
    1. A 0.1% (v/v) formic acid
    2. B acetonitrile 0.1% (v/v) formic acid
    3. The gradient was started with 5% B increased to 45% B
time　（min）
time　（min）
Gradient time：600 minutes Gradient time：900 minutes

time　（min）
time　（min）

　　　　Relationship between gradient time and peak capacity

　500-1000 proteins can be identified with a gradient time of 600 minutes　





　※Data provided by Graduate School of Agriculture, Kyoto University Assistant Professor Morisaka

Appearance and specifications are subject to change for improvement.
Please be sure to read the instruction manual before use and use it correctly.

MonoBIs Capillary Column Price list

Column sizeProduct №Price
0.1x2000mm　　LC0120　　JPY 500,000
0.1x4000mm　　LC0140　　JPY 700,000
0.075x2000mm　　LC07520　　JPY 500,000
0.075x4,000mm　　LC07540　　JPY 700,000