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 “Monolith Column” is another word for Kyoto Monotech . It is an evolutional column for liquid
chromatography which breaks the common sense of column. It has been invented by Professor
(at the time) Naohiro Soga and Professor Kazuki Nakanishi (Kyoto University), and has been developed
by Kyoto Monotech .

 What’s “Monolith Column”…?

 “Monolith” means “integrated type”. Monolith column is different from the columns packed with the conventional porous spherical particle. It is an integrated type rod-shaped column for liquid
chromatography which contains two different sizes of the pores (mesopores, macropores).  

 Monolith column uses monolithic silica gel as a separation media.  

Conventional particle packed column Monolith Column (Integrated type)



Microscope image of Monolith

   High separation
 BUT… High-back pressure

               

    High separation
 AND→ Low back pressure
    
                


When monolithic silica gel is used …

 When monolithic silica gel is used for a column of liquid chromatography, analysis can be performed with
low-back pressure at high separation effeiciency. It also enables separation of various materials such as DNA
and proteins by using a tabletop type small centrifuge or pipette in several ten seconds.
In other words:
    
 @ You can control the mesopores and macropores and silica skeleton size independently.
 A You can obtain high separation, low back pressure and easy operation at a same time.
 B We will aim to simplify and cost reduction of the separation process.

The stracture of monolithic silica gel …     

 When you look at the cross-section of a monolith silica gel in the SEM photograph, you can see two types of pores, the nanometer (nm) size pores (mesopores) and micrometer (μm) size pores (macropores).



          mesopores in skeleton                macropores


                                

                  




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